2 edition of Genetic analysis of geminivirus systemic spread and symptom induction. found in the catalog.
Genetic analysis of geminivirus systemic spread and symptom induction.
Albrecht G. von Arnim
Thesis (Ph.D.), University of East Anglia, School of Biological Sciences, 1991.
Chu M., Desvoyes B., Turina M., Noad R., Scholthof H. B. ; Genetic dissection of tomato bushy stunt virus pprotein-mediated host-dependent symptom induction and systemic Cited by: The Philadelphia chromosome or Philadelphia translocation (Ph) is a specific genetic abnormality in chromosome 22 of leukemia cancer cells (particularly chronic myeloid leukemia (CML) cells). This chromosome is defective and unusually short because of reciprocal translocation, t(9;22)(q34;q11), of genetic material between chromosome 9 and chromos and contains a fusion gene called Specialty: Oncology. Cassava (Manihot esculenta Crantz) is the fourth most important source of carbohydrates for human consumption in the tropics and thus occupies a uniquely important position as a food security crop for smallholder farmers. Consequently, cassava improvement is of high priority to most national agricultural research institutions in the tropics. With advances in functional genomics and genome Cited by: 1. Salmonella is a genus of rod-shaped (bacillus) Gram-negative bacteria of the family two species of Salmonella are Salmonella enterica and Salmonella bongori. S. enterica is the type species and is further divided into six subspecies that include over 2, serotypes. Salmonella was named after Daniel Elmer Salmon (–), an American veterinary : Gammaproteobacteria.
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Genetic analysis of geminivirus systemic spread and symptom induction. (Thesis) Arnim AGv. Publisher: University of East Anglia  Metadata Source: The British Library Type: Thesis. Abstract. No abstract supplied.
Menu. Formats. Abstract. EThOS. About. About Europe PMC Author: Arnim AGv. Genetic analysis of geminivirus systemic spread and symptom induction Author: Arnim, Albrecht G. von.
Topics: 06V - Genetics, cytology, molecular biology, 06M - Microbiology, 06W - Botany, Genetic analysis of geminivirus systemic spread and symptom induction [ Plant viruses infection cycle]Author: A.G.
von Arnim. Determinants of tissue tropism in animal-infecting viruses have been extensively investigated, but little is known about plant viruses in this regard.
Some geminiviruses in the genus Begomovirus exhibit phloem limitation and are restricted to cells of the vascular system, whereas others can invade mesophyll by: Variations in the length of the period between virus inoculation and the onset of symptoms (defined as the latent period) and in symptom severity suggest distinct levels of virus adaptation to the.
Abstract. Tomato yellow leaf curl virus (TYLCV) is a whitefly-transmitted geminivirus with a monopartite genome. We have investigated the functions of the V1, V2, and C2 ORFs by mutational analysis. We analyzed the ability of TYLCV mutants containing disrupted ORFs V1, V2, or C2 to replicate, spread, and cause symptoms in Nicotiana benthamiana Cited by: In these instances, symptoms are more pronounced than infections where only the A DNA is agroinoculated.
This is the first report of a whitefly-transmitted dicot-infecting geminivirus capable of infection (via agroinoculation), symptom induction, and systemic movement using a single by: Other mRNAs produced by the CaLCuV‐derived vector may be similarly targeted, even in a wild‐type geminivirus infection.
Reduced symptoms are often associated with later stages of geminivirus infection (Hormuzdi and Bisaro, ), and siRNAs from geminivirus pathogens have been detected (Lucioli et al., Genetic analysis of geminivirus systemic spread and symptom induction.
book even in silenced tissue Cited by: Differential roles of geminivirus Rep and AC 4(C 4) in the induction of necrosis in associated with the induction of local necrosis and a systemic burst of hydrogen peroxide production, when.
Geminivirus Replication kD kbp kD kD REn Figure I Geminivirus genome organization. The diagrams depict the double- stranded replicative forms of maize streak virus (MSV, subgroup I), beet curly top virus (BCTV, subgroup II), tomato yellow leaf curl virus (TYLCV, mono- partite, subgroup III), and tomato golden mosaic virus (TGMV, A and B.
This book provides in-depth information on all key aspects of geminivirus biology, e.g. the genetics and evolution, global diversity and spread of these plant pathogens, as well as the molecular mechanisms underlying their virulence.
Viral long distant transport is an essential step for systemic infection. Because the process involves different types of highly differentiated vascular-associated cells, the virus systemic movement is regulated differentially at each tissue by: Tomato golden mosaic virus is a bipartite geminivirus whose genome is divided between two circular DNA molecules.
DNA A encodes functions necessary for viral DNA replication and encapsidation, whereas DNA B provides functions needed for movement in the by: Genetic Analysis of Bipartite Geminivirus Tissue Tropism Ying Qin1 and Ian T. Petty2 Department of Microbiology, North Carolina State University, Raleigh, North Carolina Received J ; returned to author for revision September 3, ; accepted Septem Systemic Acquired Resistance (SAR) Another form of strong disease resistance in plants is systemic acquired re-sistance, or SAR.
Infection by a necrotizing pathogen (i.e. a pathogen that causes host cell death) causes a signal to be transmitted throughout the plant.
In response, defense genes are activated in uninfected tissue, and the plant. Goals / Objectives The overall goal of this project is to identify, understand and manage plant diseases caused by geminiviruses in California, the United States and worldwide. Geminiviruses are an emerging group of single-stranded DNA viruses that cause economically important diseases of vegetable crops including cotton, cucurbits.
Maize streak virus genes essential for systemic spread and symptom development. This article has been cited by other articles in PMC. Abstract. The entire genome of single component geminiviruses such as maize streak virus (MSV) consists of a single-stranded circular DNA of ~ by: CRISPR/Cas DNA editing system was most recently developed as a new method for genome engineering.
It is based on the type II CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR associated) immune system in bacteria that protects against invading DNA viruses and/or by: Curtovirus movement has been much less studied compared with that of other geminiviruses.
A genetic analysis of BCTV genes showed that the V3 ORF encodes the MP (Hormuzdi and Bisaro, ), which forms vesicle‐like structures that co‐localize with the endoplasmic reticulum (ER) and are trafficked intracellularly from the nucleus to the cell Cited by: Abstract. Without the equivalent of a copper or fungicide spray, virtually all realistic control measures for plant virus diseases in the field fall into two categories (Fraser, ), (i) preventing the virus and the plant from coming in contact in a manner that can initiate an infection (Chapter 14), and (ii) biologically based interference with virus replication, spread or symptom by: However, the contribution of other geminivirus proteins to the induction and circumvention of the host defence response remains to be investigated.
Geminiviruses belong to a unique family of circular single‐stranded DNA viruses that replicate by a rolling circle mechanism in the plant cell nucleus (reviewed by Hanley‐Bowdoin et al., ).Cited by: Geminiviruses infect numerous different plants, and new strains emerge often, as the virus swaps genetic material with other strains.
The spread of infection is limited to the ecosystems where the carrier insects live, which tend to be temperate climates that are also good for growing crops. (source: Moffat) References.
Updated June 5, Purpose. Stillbirth is one of the most common adverse pregnancy outcomes, occurring in 1 in deliveries in the United States. Approximat stillbirths at 20 weeks or greater of gestation are reported annually purpose of this document is to review the current information on stillbirth, including definitions and management, the evaluation of a stillbirth, and strategies for.
Geminiviruses are single-stranded DNA viruses that infect a broad range of dicotyledonous and monocotyledonous plants.
Viruses of the family Geminiviridae have been classified into four genera based on host range and genome organization ().Of these, the whitefly-transmitted geminiviruses (genus Begomovirus) are the largest group, with more than virus species. Non-cell autonomous RNA silencing can spread from cell to cell and over long distances in animals and plants.
However, the genetic requirements and signals involved in plant mobile gene silencing are poorly understood. Here, we identified a DICER-LIKE2 (DCL2) - dependent mechanism for systemic spread of posttranscriptional RNA silencing, also known as posttranscriptional gene Cited by: Inoculation of turnip crinkle virus (TCV) on the resistant Arabidopsis ecotype Dijon (Di) results in the development of a hypersensitive response (HR) on the inoculated leaves.
To assess the role of the recently cloned HRT gene in conferring resistance, we monitored both HR and resistance (lack of viral spread to systemic tissues) in the progeny of a cross between resistant Di and Cited by: When coresident with the Ti (tumor-inducing) plasmid, the kDa product of the osa gene of the plasmid pSa can suppress crown gall tumorigenesis incited by Agrobacterium tumefaciens.
Neither T-DNA processing nor vir (virulence) gene induction is affected by the presence of osa in the bacterium. We used Arabidopsis thaliana root segments and tobacco leaf discs to demonstrate that Cited by: Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions.
However, J. curcus is susceptible to the geminivirus Indian cassava mosaic virus (ICMV), and frequent viral disease outbreaks severely limit productivity.
Therefore the development of J. curcus to carry on durable virus resistance remains crucial and poses a major biotechnological Cited by: Interest in interstitial lung diseases (ILDs) has risen in recent years.
A large volume of basic and clinical research has increased our understanding of the pathogenesis of idiopathic pulmonary fibrosis (IPF) and non-IPF fibrotic ILDs. The ILD field is now evolving rapidly, with major implications for practical management.
This Monograph provides expert clinical guidance on these difficult Author: Wim A. Wuyts, Vincent Cottin, Paolo Spagnolo, Athol U. Wells. Some geminiviruses have acquired a satellite called DNA β (betasatellite), which depends on the helper virus for replication and spread within and between hosts.
In return, the sole betasatellite encoded protein, βC1, encodes a pathogenicity factor that enhances viral replication and is responsible for inducing disease by: A.3 Virus Diseases Chapter 22 Geminiviruses 2 Eupatorium odoratum, Euphorbia hirta, Nicotiana benthamiana, Lunicera spp., Sida rhombifolia, Solanum nigrum, Veronica cinerea and Withania somnifera (Ch.
61). Resistant varieties. Many studies on development of resistant tobacco lineeminivirus have been dones to g. Gene silencing is proving to be a powerful tool for genetic, developmental, and physiological analyses. The use of viral induced gene silencing (VIGS) offers advantages to transgenic approaches as it can be potentially applied to non-model systems for which transgenic techniques are not readily available.
However, many VIGS vectors are derived from Gemini viruses that have limited Cited by: Geminivirus AC2 is a multifunctional protein that acts as a pathogenicity factor. Transcriptional regulation by AC2 appears to be mediated through interaction with a plant specific DNA binding protein, PEAPOD2 (PPD2), that specifically binds to sequences known to mediate activation of the CP promoter of Cabbage leaf curl virus (CaLCuV) and Tomato golden mosaic virus (TGMV).Cited by: 8.
Genes involved in a viral resistance response in common bean (Phaseolus vulgaris cv. Othello) were identified by inoculating a geminivirus reporter (Bean dwarf mosaic virus expressing the green fluorescent protein), extracting RNA from tissue undergoing the defense response, and amplifying sequences with degenerate R gene primers.
One such gene (a TIR-NBS-LRR gene, RT) was Cited by: Plants with the novel symptoms were also analyzed for the presence of other RNA or DNA viruses, fungi, bacteria and other stresses.
Copper sulfate was added to remove any fungi. A growth chamber in the NCSU Plant Transformation Lab, which is convenient to our laboratory, was also rented and used to grow plants. Background: Tomato Yellow Leaf Curl Virus (TYLCV) is a circular single strand DNA begomovirus, which causes one of the most important diseases in tomato worldwide.
This virus was observed on tomato plants for first time in Saudi Arabia in and has been endemic since then. Materials and Methods: Plant samples of pepper (Capsicum frutescens) and common bean (showing leaf yellowing and.
Despite the non-coding nature of their small RNA genomes, the visible symptoms of viroid infection resemble those associated with many plant virus diseases. Recent evidence indicates that viroid-derived small RNAs acting through host RNA silencing pathways play a key role in viroid pathogenicity.
Host responses to viroid infection are complex, involving signaling cascades containing host Cited by: PHYVV and PepGMV are plant viruses reported in Mexico and Southern US as causal agents of an important pepper disease known as "rizado amarillo".
Mixed infections with PHYVV and PepGMV have been reported in several hosts over a wide geographic area. Previous work suggested that these viruses might interact at the replication and/or movement level in a complex manner. The Cited by: Analysis of expression of the reporter transgene in the F1 progeny can also reveal whether the VSR suppresses local silencing, DNA methylation of the reporter transgene, or both.
Viruses are able to spread through infected plant cells using two ways of movement: cell Cited by: 3. Central nervous system (CNS) metastases rarely occur in patients with neuroblastoma, although recent reports suggest an increase in the rate.
CNS recurrence may represent a different mechanism of spread than bone and bone marrow metastases and may be associated with unique genetic determinants. The tomato leaf curl New Delhi virus (ToLCNDV) (genus Begomovirus, family Geminiviridae) represents an important constraint to tomato production, as it causes the most predominant and economically important disease affecting tomato in the Indian sub-continent.
However, in recent years, ToLCNDV has been fast extending its host range and spreading to new geographical regions, including the Cited by: Lu, H. et al. Genetic analysis of acd reveals complex defense networks and leads to identification of novel defense genes in Arabidopsis.
Plant J. 58, – () CASCited by: Cloned DNA-A and DNA-B components of Kenyan isolates of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2), East African cassava mosaic Kenya virus (EACMKV) and East African cassava mosaic Zanzibar virus (EACMZV) are shown to be infectious in cassava.
EACMV and EACMKV genomic components have the same iteron sequence (GGGGG) and can form viable Cited by: